Engineering terpenoid production through transient expression in Nicotiana benthamiana
نویسندگان
چکیده
منابع مشابه
Transient Expression of Tetrameric Recombinant Human Butyrylcholinesterase in Nicotiana benthamiana
To optimize the expression, extraction and purification of plant-derived tetrameric recombinant human butyrylcholinesterase (prBChE), we describe the development and use of plant viral amplicon-based gene expression system; Tobacco Mosaic Virus (TMV) RNA-based overexpression vector (TRBO) to express enzymatically active FLAG-tagged plant made recombinant butyrylcholinesterase (rBChE) in Nicotia...
متن کاملProduction of the Main Celiac Disease Autoantigen by Transient Expression in Nicotiana benthamiana
Celiac Disease (CD) is a gluten sensitive enteropathy that remains widely undiagnosed and implementation of massive screening tests is needed to reduce the long term complications associated to untreated CD. The main CD autoantigen, human tissue transglutaminase (TG2), is a challenge for the different expression systems available since its cross-linking activity affects cellular processes. Plan...
متن کاملTechno-economic analysis of horseradish peroxidase production using a transient expression system in Nicotiana benthamiana.
Despite the advantages of plant-based transient expression systems relative to microbial or mammalian cell systems, the commercial production of recombinant proteins using plants has not yet been achieved to any significant extent. One of the challenges has been the lack of published data on the costs of manufacture for products other than biopharmaceuticals. In this study, we report on the tec...
متن کاملEngineering of human-type O-glycosylation in Nicotiana benthamiana plants
Therapeutic properties of recombinant proteins are very often affected by the composition and heterogeneity of their glycans. Conventional expression systems for recombinant pharmaceutical proteins typically do not address this problem and produce a mixture of glycoforms that are neither identical to human glycans nor optimized for enhanced efficacy. In terms of glycosylation, plants offer cert...
متن کاملA terpenoid phytoalexin plays a role in basal defense of Nicotiana benthamiana against Potato virus X
Terpenoid phytoalexins function as defense compound against a broad spectrum of pathogens and pests in the plant kingdom. However, the role of phytoalexin in antiviral defense is still elusive. In this study, we identified the biosynthesis pathway of a sesquiterpenoid phytoalexin, capsidiol 3-acetate as an antiviral response against RNA virus Potato Virus X (PVX) in Nicotiana benthamiana. NbTPS...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Cell Reports
سال: 2018
ISSN: 0721-7714,1432-203X
DOI: 10.1007/s00299-018-2296-3